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odyssey infrared imaging system  (LI-COR)


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    Structured Review

    LI-COR odyssey infrared imaging system
    Odyssey Infrared Imaging System, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 7143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/odyssey+infrared+imaging+system/Odyssey+FC+Imaging+System/pmc13625450-189-6-10
    Average 99 stars, based on 7143 article reviews
    odyssey infrared imaging system - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Infrared:

    Article Title: Anti-plasmodial activity of proteasome inhibitors against various Plasmodium species: a preclinical evaluation.
    Article Snippet: Membranes were blocked with 5% skim milk at RT for 1 h and probed with mouse anti-ubiquitin antibodies (dilution 1/500 in blocking buffer; Santa Cruz Biotechnology) or mouse anti-GAPDH (dilution 1/5,000 in blocking buffer; Sigma‒Aldrich), followed by goat anti-mouse IRDye (dilution 1/10,000 in blocking buffer; Invitrogen). .. Signals were visualized using an Odyssey infrared imaging system (LI-COR Bioscience), quantified by densitometry using software (Image J2), and the ubiquitin signal was normalized to GAPDH. ..

    Article Title: The protein kinase IKK epsilon shapes the melanoma tumor microenvironment by modulating angiogenesis and the T-cell immunity
    Article Snippet: .. Finally, gels were scanned using the Odyssey Infrared Imaging System (LI-COR Biosciences), and band intensities were quantified with Image Studio Lite Software V6.0 (LI-COR Biosciences, Bad Homburg, Germany). .. RNA-isolation from harvested HUVEC was performed using the Qiagen RNeasy Mini Kit (Qiagen, Hilden, Germany, #74106) according to the manufacturer ́s instructions.

    Article Title: A hypothalamic neuronal population coordinates metabolic adaptations to cold
    Article Snippet: After washing, membranes were incubated for 1 hour with Alexa Fluor Plus 680-conjugated (A32729, Invitrogen) or 800-conjugated (A32735, Invitrogen) secondary antibodies. .. Fluorescent signals were detected using the Odyssey Infrared Imaging System (LI-COR Biotechnology), and band intensities were quantified using ImageJ software. ..

    Article Title: Cell-free hemoglobin upregulates pulmonary endothelial heparanase expression to drive glycocalyx destruction and inflammation in sepsis.
    Article Snippet: Blots were washed five times in 0.1% TBS-T and incubated with goat anti-rabbit secondary (1:15,000; 926-32211, LI-COR) for 1 hour at 25°C. .. Visualization was completed with the Odyssey infrared imaging system (LI-COR). ..

    Article Title: HSV-1 UL42 and UL47 hijack cellular nucleotide synthetic enzyme CAD to promote lytic replication.
    Article Snippet: All immunoblotting was performed using the following primary antibodies: RelA (Invitrogen, PA5-16545, 1:2,000 dilution), CAD (Bethyl Laboratories, A301-374A, 1:2,000 dilution), Phospho-CAD (Ser1859) (Cell Signaling, 12662, 1:1,000 dilution), Flag (Sigma, F3165, 1:1,000 dilution), and V5 (Bethyl Laboratories, A190-120A, 1:1,000 dilution). .. Proteins were visualized by Odyssey infrared imaging system (LI-COR) after incubation with IRDye800-conjugated secondary antibodies (1:10,000 dilution, LI-COR). ..

    Article Title: TSPO deficiency uncovers a novel role for porphyrins in adipocyte lipid metabolism
    Article Snippet: After washing with TBS-T, membranes were incubated with fluorescently labeled secondary antibodies: IRDye 800CW goat anti-rabbit IgG and IRDye 680RD goat anti-mouse IgG (Li-Cor). .. Fluorescent signals were detected using an Odyssey infrared imaging system (Li-Cor). .. Selected membranes were processed for Coomassie blue staining (Thermo Scientific) and follow-up imaging to assess overall protein distribution across lanes.

    Article Title: Interleukin-17A directly triggers Epstein-Barr virus lytic reactivation in latency I human B-cell models.
    Article Snippet: Signals were acquired using a Bio-Rad ChemiDoc imaging system or by exposure to Fuji Super RX films (Fujifilm), followed by film scanning. .. To detect infrared fluorescence signals, an Odyssey infrared imaging system (Li-Cor Biosciences) was used with a secondary antibody (IRDye 680RD goat anti-rabbit antibody; Li-Cor, Cat# 925-68071). ..

    Article Title: Mutant p53 R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation
    Article Snippet: Membranes were blocked with 5% nonfat milk in PBS containing 0.1% Tween‐20 and incubated with antibodies [mouse anti‐FLAG (Sigma‐Aldrich, #F1804), mouse anti‐p53 (Santa Cruz Biotechnology, #sc‐126), rabbit anti‐p53 (Santa Cruz Biotechnology, #sc‐6243), rabbit anti‐PDPK1 (Cell Signaling Technology, #3062), rabbit anti‐AKT (Cell Signaling Technology, #9272), rabbit anti‐phospho‐AKT (T308) (Cell Signaling Technology, #13038), mouse anti‐Actin (Merck Millipore, #MAB1501), mouse anti‐GST (Santa Cruz Biotechnology, #sc‐138), and rabbit anti‐MYC (Proteintech, #16286‐1‐AP)]. .. Detection of blots was done with ECL Prime Western Blotting Detection Reagent (Cytiva) for goat anti‐mouse or anti‐rabbit secondary antibodies conjugated with horseradish peroxidase (HRP) (Jackson ImmunoResearch) or done with Odyssey Infrared Imaging System (LI‐COR Biosciences) for DyLight 680‐ or 800‐fluorescent‐conjugated goat anti‐mouse or anti‐rabbit secondary antibodies (Thermo Fisher Scientific). .. Small interfering RNA (siRNA) targeting TP53 #1 (forward: GUAAUCUACUGGGACGGAAdTdT, reverse: UUCCGUCCCAGUAGAUUACdCdA), TP53 #2 (forward: GGUGAACCUUAGUACCUAAdTdT, reverse: UUAGGUACUAAGGUUCACCdAdA), PDPK1 #1 (forward: GCGGUUAGGCUGUGAGGAAdTdT, reverse: UUCCUCACAGCCUAACCGCdTdT), PDPK1 #2 (forward: CGCCUAACAGGACGUAUUAdTdT, reverse: UAAUACGUCCUGUUAGGCGdTdG), or control siRNA (Qiagen, 1027310) were transfected into cells at a final concentration of 40 nM using the calcium phosphate transfection protocol as previously described [ ].

    Imaging:

    Article Title: Anti-plasmodial activity of proteasome inhibitors against various Plasmodium species: a preclinical evaluation.
    Article Snippet: Membranes were blocked with 5% skim milk at RT for 1 h and probed with mouse anti-ubiquitin antibodies (dilution 1/500 in blocking buffer; Santa Cruz Biotechnology) or mouse anti-GAPDH (dilution 1/5,000 in blocking buffer; Sigma‒Aldrich), followed by goat anti-mouse IRDye (dilution 1/10,000 in blocking buffer; Invitrogen). .. Signals were visualized using an Odyssey infrared imaging system (LI-COR Bioscience), quantified by densitometry using software (Image J2), and the ubiquitin signal was normalized to GAPDH. ..

    Article Title: The protein kinase IKK epsilon shapes the melanoma tumor microenvironment by modulating angiogenesis and the T-cell immunity
    Article Snippet: .. Finally, gels were scanned using the Odyssey Infrared Imaging System (LI-COR Biosciences), and band intensities were quantified with Image Studio Lite Software V6.0 (LI-COR Biosciences, Bad Homburg, Germany). .. RNA-isolation from harvested HUVEC was performed using the Qiagen RNeasy Mini Kit (Qiagen, Hilden, Germany, #74106) according to the manufacturer ́s instructions.

    Article Title: A hypothalamic neuronal population coordinates metabolic adaptations to cold
    Article Snippet: After washing, membranes were incubated for 1 hour with Alexa Fluor Plus 680-conjugated (A32729, Invitrogen) or 800-conjugated (A32735, Invitrogen) secondary antibodies. .. Fluorescent signals were detected using the Odyssey Infrared Imaging System (LI-COR Biotechnology), and band intensities were quantified using ImageJ software. ..

    Article Title: Cell-free hemoglobin upregulates pulmonary endothelial heparanase expression to drive glycocalyx destruction and inflammation in sepsis.
    Article Snippet: Blots were washed five times in 0.1% TBS-T and incubated with goat anti-rabbit secondary (1:15,000; 926-32211, LI-COR) for 1 hour at 25°C. .. Visualization was completed with the Odyssey infrared imaging system (LI-COR). ..

    Article Title: HSV-1 UL42 and UL47 hijack cellular nucleotide synthetic enzyme CAD to promote lytic replication.
    Article Snippet: All immunoblotting was performed using the following primary antibodies: RelA (Invitrogen, PA5-16545, 1:2,000 dilution), CAD (Bethyl Laboratories, A301-374A, 1:2,000 dilution), Phospho-CAD (Ser1859) (Cell Signaling, 12662, 1:1,000 dilution), Flag (Sigma, F3165, 1:1,000 dilution), and V5 (Bethyl Laboratories, A190-120A, 1:1,000 dilution). .. Proteins were visualized by Odyssey infrared imaging system (LI-COR) after incubation with IRDye800-conjugated secondary antibodies (1:10,000 dilution, LI-COR). ..

    Article Title: TSPO deficiency uncovers a novel role for porphyrins in adipocyte lipid metabolism
    Article Snippet: After washing with TBS-T, membranes were incubated with fluorescently labeled secondary antibodies: IRDye 800CW goat anti-rabbit IgG and IRDye 680RD goat anti-mouse IgG (Li-Cor). .. Fluorescent signals were detected using an Odyssey infrared imaging system (Li-Cor). .. Selected membranes were processed for Coomassie blue staining (Thermo Scientific) and follow-up imaging to assess overall protein distribution across lanes.

    Article Title: Interleukin-17A directly triggers Epstein-Barr virus lytic reactivation in latency I human B-cell models.
    Article Snippet: Signals were acquired using a Bio-Rad ChemiDoc imaging system or by exposure to Fuji Super RX films (Fujifilm), followed by film scanning. .. To detect infrared fluorescence signals, an Odyssey infrared imaging system (Li-Cor Biosciences) was used with a secondary antibody (IRDye 680RD goat anti-rabbit antibody; Li-Cor, Cat# 925-68071). ..

    Article Title: Mutant p53 R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation
    Article Snippet: Membranes were blocked with 5% nonfat milk in PBS containing 0.1% Tween‐20 and incubated with antibodies [mouse anti‐FLAG (Sigma‐Aldrich, #F1804), mouse anti‐p53 (Santa Cruz Biotechnology, #sc‐126), rabbit anti‐p53 (Santa Cruz Biotechnology, #sc‐6243), rabbit anti‐PDPK1 (Cell Signaling Technology, #3062), rabbit anti‐AKT (Cell Signaling Technology, #9272), rabbit anti‐phospho‐AKT (T308) (Cell Signaling Technology, #13038), mouse anti‐Actin (Merck Millipore, #MAB1501), mouse anti‐GST (Santa Cruz Biotechnology, #sc‐138), and rabbit anti‐MYC (Proteintech, #16286‐1‐AP)]. .. Detection of blots was done with ECL Prime Western Blotting Detection Reagent (Cytiva) for goat anti‐mouse or anti‐rabbit secondary antibodies conjugated with horseradish peroxidase (HRP) (Jackson ImmunoResearch) or done with Odyssey Infrared Imaging System (LI‐COR Biosciences) for DyLight 680‐ or 800‐fluorescent‐conjugated goat anti‐mouse or anti‐rabbit secondary antibodies (Thermo Fisher Scientific). .. Small interfering RNA (siRNA) targeting TP53 #1 (forward: GUAAUCUACUGGGACGGAAdTdT, reverse: UUCCGUCCCAGUAGAUUACdCdA), TP53 #2 (forward: GGUGAACCUUAGUACCUAAdTdT, reverse: UUAGGUACUAAGGUUCACCdAdA), PDPK1 #1 (forward: GCGGUUAGGCUGUGAGGAAdTdT, reverse: UUCCUCACAGCCUAACCGCdTdT), PDPK1 #2 (forward: CGCCUAACAGGACGUAUUAdTdT, reverse: UAAUACGUCCUGUUAGGCGdTdG), or control siRNA (Qiagen, 1027310) were transfected into cells at a final concentration of 40 nM using the calcium phosphate transfection protocol as previously described [ ].

    Software:

    Article Title: Anti-plasmodial activity of proteasome inhibitors against various Plasmodium species: a preclinical evaluation.
    Article Snippet: Membranes were blocked with 5% skim milk at RT for 1 h and probed with mouse anti-ubiquitin antibodies (dilution 1/500 in blocking buffer; Santa Cruz Biotechnology) or mouse anti-GAPDH (dilution 1/5,000 in blocking buffer; Sigma‒Aldrich), followed by goat anti-mouse IRDye (dilution 1/10,000 in blocking buffer; Invitrogen). .. Signals were visualized using an Odyssey infrared imaging system (LI-COR Bioscience), quantified by densitometry using software (Image J2), and the ubiquitin signal was normalized to GAPDH. ..

    Article Title: The protein kinase IKK epsilon shapes the melanoma tumor microenvironment by modulating angiogenesis and the T-cell immunity
    Article Snippet: .. Finally, gels were scanned using the Odyssey Infrared Imaging System (LI-COR Biosciences), and band intensities were quantified with Image Studio Lite Software V6.0 (LI-COR Biosciences, Bad Homburg, Germany). .. RNA-isolation from harvested HUVEC was performed using the Qiagen RNeasy Mini Kit (Qiagen, Hilden, Germany, #74106) according to the manufacturer ́s instructions.

    Ubiquitin Proteomics:

    Article Title: Anti-plasmodial activity of proteasome inhibitors against various Plasmodium species: a preclinical evaluation.
    Article Snippet: Membranes were blocked with 5% skim milk at RT for 1 h and probed with mouse anti-ubiquitin antibodies (dilution 1/500 in blocking buffer; Santa Cruz Biotechnology) or mouse anti-GAPDH (dilution 1/5,000 in blocking buffer; Sigma‒Aldrich), followed by goat anti-mouse IRDye (dilution 1/10,000 in blocking buffer; Invitrogen). .. Signals were visualized using an Odyssey infrared imaging system (LI-COR Bioscience), quantified by densitometry using software (Image J2), and the ubiquitin signal was normalized to GAPDH. ..

    Incubation:

    Article Title: HSV-1 UL42 and UL47 hijack cellular nucleotide synthetic enzyme CAD to promote lytic replication.
    Article Snippet: All immunoblotting was performed using the following primary antibodies: RelA (Invitrogen, PA5-16545, 1:2,000 dilution), CAD (Bethyl Laboratories, A301-374A, 1:2,000 dilution), Phospho-CAD (Ser1859) (Cell Signaling, 12662, 1:1,000 dilution), Flag (Sigma, F3165, 1:1,000 dilution), and V5 (Bethyl Laboratories, A190-120A, 1:1,000 dilution). .. Proteins were visualized by Odyssey infrared imaging system (LI-COR) after incubation with IRDye800-conjugated secondary antibodies (1:10,000 dilution, LI-COR). ..

    Fluorescence:

    Article Title: Interleukin-17A directly triggers Epstein-Barr virus lytic reactivation in latency I human B-cell models.
    Article Snippet: Signals were acquired using a Bio-Rad ChemiDoc imaging system or by exposure to Fuji Super RX films (Fujifilm), followed by film scanning. .. To detect infrared fluorescence signals, an Odyssey infrared imaging system (Li-Cor Biosciences) was used with a secondary antibody (IRDye 680RD goat anti-rabbit antibody; Li-Cor, Cat# 925-68071). ..

    Western Blot:

    Article Title: Mutant p53 R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation
    Article Snippet: Membranes were blocked with 5% nonfat milk in PBS containing 0.1% Tween‐20 and incubated with antibodies [mouse anti‐FLAG (Sigma‐Aldrich, #F1804), mouse anti‐p53 (Santa Cruz Biotechnology, #sc‐126), rabbit anti‐p53 (Santa Cruz Biotechnology, #sc‐6243), rabbit anti‐PDPK1 (Cell Signaling Technology, #3062), rabbit anti‐AKT (Cell Signaling Technology, #9272), rabbit anti‐phospho‐AKT (T308) (Cell Signaling Technology, #13038), mouse anti‐Actin (Merck Millipore, #MAB1501), mouse anti‐GST (Santa Cruz Biotechnology, #sc‐138), and rabbit anti‐MYC (Proteintech, #16286‐1‐AP)]. .. Detection of blots was done with ECL Prime Western Blotting Detection Reagent (Cytiva) for goat anti‐mouse or anti‐rabbit secondary antibodies conjugated with horseradish peroxidase (HRP) (Jackson ImmunoResearch) or done with Odyssey Infrared Imaging System (LI‐COR Biosciences) for DyLight 680‐ or 800‐fluorescent‐conjugated goat anti‐mouse or anti‐rabbit secondary antibodies (Thermo Fisher Scientific). .. Small interfering RNA (siRNA) targeting TP53 #1 (forward: GUAAUCUACUGGGACGGAAdTdT, reverse: UUCCGUCCCAGUAGAUUACdCdA), TP53 #2 (forward: GGUGAACCUUAGUACCUAAdTdT, reverse: UUAGGUACUAAGGUUCACCdAdA), PDPK1 #1 (forward: GCGGUUAGGCUGUGAGGAAdTdT, reverse: UUCCUCACAGCCUAACCGCdTdT), PDPK1 #2 (forward: CGCCUAACAGGACGUAUUAdTdT, reverse: UAAUACGUCCUGUUAGGCGdTdG), or control siRNA (Qiagen, 1027310) were transfected into cells at a final concentration of 40 nM using the calcium phosphate transfection protocol as previously described [ ].



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